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1.00213

Millipore

NutriSelect® prime Sorbitol MacConkey (SMAC) Agar

acc. FDA-BAM, ISO 16654

Synonym(s):

CT-SMAC Agar, MacConkey Agar with Sorbitol, SMAC Agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

FDA-BAM

Quality Level

200

form

powder

shelf life

5 yr

composition

  • Enzymatic digest of casein 17.0 g/L
  • Enzymatic digest of animal tissue 3.0 g/L
  • Sodium chloride 5.0 g/L
  • Bile salts No. 3 1.5 g/L
  • Sorbitol 10.0 g/L
  • Neutral red 0.03 g/L
  • Crystal violet 0.001 g/L
  • Agar-agar 15.0 g/L

packaging

container of 500 g

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.1±0.2 (25 °C)

solubility

water: soluble

application(s)

food and beverages
pharmaceutical

compatibility

for use with CT supplement (77981)

shipped in

ambient

storage temp.

15-25°C

suitability

selective and differential for Escherichia 0157:H7

General description

GranuCult® prime Sorbitol MacConkey (SMAC) Agar is a selective agar medium to isolate and differentiate enterohemorrhagic (EHEC) E. coli 0157 from food, animal feed and other samples.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.

Application

GranuCult® prime Sorbitol MacConkey (SMAC) Agar is used for the isolation and differentiation of sorbitol negative Escherichia coli serotype O157 from food, animal feed and other test samples.

Analysis Note

  • The dehydrated media is granulated with light pink color.
  • Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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