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M7522

Sigma-Aldrich

2-Mercaptoethanol

liquid, BioReagent, suitable for cell culture

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
Beilstein:
773648
EC Number:
200-464-6
MDL number:
MFCD00004890
UNSPSC Code:
12352200
PubChem Substance ID:
24897168

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioReagent

form

liquid

expl. lim.

18 %

concentration

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable

refractive index

n20/D 1.500 (lit.)

bp

157 °C (lit.)

solubility

H2O: 1 mL/mL

density

1.114 g/mL at 25 °C (lit.)

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Target Organs

Liver,Heart

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

165.2 °F - closed cup

Flash Point(C)

74 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
01496DK
42896BP
42896BPV
BCBD8822V
BCBD2112V

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Karl W Boehme et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(47), 19986-19991 (2009-11-10)
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Annals of the New York Academy of Sciences, 1176, 219-227 (2009-10-03)
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Manching Ku et al.
PLoS genetics, 4(10), e1000242-e1000242 (2008-11-01)
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of
Vesna Nikolova-Krstevski et al.
BMC genomics, 9, 240-240 (2008-05-24)
Endothelial differentiation occurs during normal vascular development in the developing embryo. This process is recapitulated in the adult when endothelial progenitor cells are generated in the bone marrow and can contribute to vascular repair or angiogenesis at sites of vascular
Maria Bettini et al.
Journal of neuroimmunology, 213(1-2), 60-68 (2009-06-23)
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