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SAB4200801

Sigma-Aldrich

Anti-Rabbit IgG (γ-chain specific) antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

Synonym(s):

Anti-Rabbit immunoglobulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RG-96, monoclonal

form

buffered aqueous solution

species reactivity

rabbit

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: suitable
immunohistochemistry: suitable
indirect ELISA: 0.2-0.4 μg/mL using 10 μg/mL rabbit IgG for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Primary Rabbit antibodies are widely used in research for various assay techniques. Secondary antibodies that are used to detect rabbit antibodies may lack specificity for rabbit immunoglobulins and in many instances will also recognize other immunoglobulins that appear in the sample being tested. This results rise the need for extensive adsorption of the second antibody. Therefore, using a monoclonal antibody to rabbit IgG which is devoid of a binding capacity to other species can serve as a useful research tool in many applications. Monoclonal Anti-Rabbit IgG (γ-chain specific) recognizes an epitope located on the heavy γ-chain of rabbit IgG. The antibody does not react with rabbit IgA, IgM or light chains. No cross reactivity is observed with IgG from bovine, cat, chicken, dog, goat, horse, human, pig or sheep.

Immunogen

purified rabbit IgG

Application

The antibody is recommended to use in various immunological techniques, including ELISA, Immunoblotting and Immunohistochemistry.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
058M4820
51180216

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Changyu Huang et al.
BMC immunology, 24(1), 55-55 (2023-12-22)
The interaction between the nervous system and the immune system can affect the outcome of a bacterial infection. Staphylococcus aureus skin infection is a common infectious disease, and elucidating the relationship between the nervous system and immune system may help

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