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NA1020

Sigma-Aldrich

GenElute™ PCR Clean-Up Kit

sufficient for 70 purifications

Synonym(s):

Gen Elute, PCR Purification

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.52

usage

sufficient for 70 purifications

technique(s)

DNA purification: suitable

storage temp.

15-25°C

General description

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Application

GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Features and Benefits

  • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
  • Recovers up to 95% of PCR products between 100 bp and 10 kb
  • Removes over 99% of primers and other components
  • Eliminates the need to remove mineral oil by organic extraction
  • 40% more purification preps supplied than market leader

Other Notes

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Principle

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290,H302,H315,H319,H336

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Charlotte G Cole et al.
Genome biology, 9(5), R78-R78 (2008-05-15)
Although the human genome sequence was declared complete in 2004, the sequence was interrupted by 341 gaps of which 308 lay in an estimated approximately 28 Mb of euchromatin. While these gaps constitute only approximately 1% of the sequence, knowledge
Adriana P Rebelo et al.
Nucleic acids research, 37(20), 6701-6715 (2009-09-11)
To characterize the organization of mtDNA-protein complexes (known as nucleoids) in vivo, we have probed the mtDNA surface exposure using site-specific DNA methyltransferases targeted to the mitochondria. We have observed that DNA methyltransferases have different accessibility to different sites on
Genetic analysis of uveal melanoma by array comparative genomic hybridization before and after radiotherapy
Werner Wackernagel
Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry, 27.6, 286-291 (2013)
Najla Chabchoub et al.
The American journal of tropical medicine and hygiene, 80(1), 24-27 (2009-01-15)
Stool samples from 86 immunocompromised patients (51 human immunodeficiency virus (HIV)-infected patients and 35 patients with haematologic malignancies) were systematically screened for intestinal microspordiosis by microscopic examination and polymerase chain reaction (PCR) using universal primer V1/PMP2. Nine samples (10.5%) showed
Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization.
Vandewoestyne M
International Journal of Cancer. Journal International Du Cancer, 130(5), 1098-1108 (2012)
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Protocols

GenElute™ PCR Clean-Up Procedure

GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.

GenElute™ PCR Clean-Up Procedure

GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.

GenElute™ PCR Clean-Up Procedure

GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.

GenElute™ PCR Clean-Up Procedure

GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.

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