GS75

GST O1-1, Recombinant Human

• Synonym(s): GSTO 1-1, GSTTLp28, HEL-S-21, P28, SPG-R, glutathione S-transferase omega 1
• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: >95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 4.21 units/mg protein
• mol wt: 26 kDa
• concentration: 1.98 mg/mL
• storage temp.: −70°C
• Gene Information: human ... GSTO1(9446)

Description

General description

using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.

Biochem/physiol Actions

Glutathione S-transferase omega 1 (GSTO1) is an enzyme that in humans is encoded by the GSTO1 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.

Storage and Stability

The enzyme should be used by the end-user customer within 1 year of receipt.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable