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C8772

Sigma-Aldrich

Cholera filtrate

lyophilized powder, bacterial sialidase receptor-destroying enzyme (RDE)

Synonym(s):

Cholera Filter

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Pricing and availability is not currently available.

Product Name

Cholera filtrate, lyophilized powder

form

lyophilized powder

Quality Level

storage temp.

2-8°C

General description

Cholera filtrate promotes the activation of adenylate cyclase. It does not affect the activity of cyclic adenosine monophosphate (cAMP) phosphodiesterase.[1]

Application

Cholerafiltrate has been used as a receptor destroying enzyme (RDE): inhemagglutination inhibition assay of serum samples[2], in microneutralizationassay of mice serum samples[3], in hemagglutination inhibition assay to removenon-specific inhibitors from the cell culture supernatant samples[4]

Biochem/physiol Actions

Cholera filtrate is a bacterial sialidase receptor-destroying enzyme (RDE) and may be used as crude neuraminidase. It may also be used in serological testing for influenza.

Reconstitution

After reconstituting with 5ml sterile water, it will contain 0.01% MIT as preservative.

Other Notes

See a separate listing of neuraminidase for preparations showing higher activity.
For research use only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins
Daniela M, et al.
Nature Communications (2024)
WHO Tech Rep. Serv.
WHO Expert Committee on Influenza, 64 (1953)
Maria Zimmermann et al.
Frontiers in immunology, 10, 829-829 (2019-05-02)
Determining antigen specificity is vital for understanding B cell biology and for producing human monoclonal antibodies. We describe here a powerful method for identifying B cells that recognize membrane antigens expressed on cells. The technique depends on two characteristics of
A V Kornev et al.
Biulleten' eksperimental'noi biologii i meditsiny, 88(10), 414-416 (1979-10-01)
An in vitro model using homogenates of the rat intestine and liver for studying the V. Cholerae culture filtrate effect on the adenylate cyclase system is proposed. Optimal conditions for the adenylate cyclase functioning have been investigated for this model.
A Riccomi et al.
Vaccines, 12(4) (2024-04-27)
A number of studies have suggested that influenza vaccination can provide protection against COVID-19, but the underlying mechanisms that could explain this association are still unclear. In this study, the effect of the 2021/2022 seasonal influenza vaccination on the immune

Questions

  1. What is the shelf life of cholera filtrate once it's reconstituted (Product No. C8772)?

    1 answer
    1. The powder has a shelf life of 4 years from the QC release date. Regarding the reconstituted product, based on real-time stability data it should be stable for up to one year, although this cannot be guaranteed. It is recommended to keep solutions in aliquots.

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