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Key Documents

A8332

Sigma-Aldrich

p-Aminobenzamidine–Agarose

(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)

Synonym(s):

pABA

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About This Item

MDL number:
MFCD00165084
UNSPSC Code:
41106514
NACRES:
NA.56

biological source

plant

form

(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)

technique(s)

protein purification: suitable

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino to carboxyl of spacer

matrix spacer

9 atoms

capacity

≥5 mg/mL binding capacity (trypsin)

storage temp.

2-8°C

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Application

p-Aminobenzamidine Agarose (pABA) can be used as an affinity ligand to bind and remove serine proteases in addition to Ca2+-binding proteins. It has also been used in medical applications as a tool for the purification of non-protease proteins.

Physical form

1:1 suspension in a 1.0 M NaCl + 20% ethanol solution

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000420139
0000420139
0000325094
0000325095
0000325095

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Gulay Bayramoglu et al.
Bioprocess and biosystems engineering, 35(3), 423-431 (2011-08-20)
In this work, a new methodology is developed for selection of affinity ligands towards the enzyme "trypsin" using quartz crystals microbalance (QCM) technique. To achieve this goal, the surface amination of gold plated QCM crystals was achieved in 13.56 MHz
Paola Fortugno et al.
Journal of cell science, 115(Pt 3), 575-585 (2002-02-28)
Survivin is a member of the inhibitor of apoptosis gene family that has been implicated in both apoptosis inhibition and regulation of mitosis. However, the subcellular distribution of survivin has been controversial and variously described as a microtubule-associated protein or
Massimo D'Agostino et al.
Nature, 551(7682), 634-638 (2017-11-02)
Membrane fusion in eukaryotic cells mediates the biogenesis of organelles, vesicular traffic between them, and exo- and endocytosis of important signalling molecules, such as hormones and neurotransmitters. Distinct tasks in intracellular membrane fusion have been assigned to conserved protein systems.
Tomas Erban
PloS one, 6(8), e22860-e22860 (2011-08-31)
p-aminobenzamidine (p-ABA) is used as a ligand in the purification of many serine proteases and in their removal from heterogeneous samples. Moreover, p-ABA has a potent ability to bind Ca(2+)-binding proteins. The binding ability and use of p-ABA in purification
Sindy Escobar-Alvarez et al.
Journal of molecular biology, 387(5), 1211-1228 (2009-02-25)
Peptide deformylase proteins (PDFs) participate in the N-terminal methionine excision pathway of newly synthesized peptides. We show that the human PDF (HsPDF) can deformylate its putative substrates derived from mitochondrial DNA-encoded proteins. The first structural model of a mammalian PDF
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