SCT084

BioTracker^™ MemBright 560 Live Cell Dye

• Synonym(s): Live cell imaging probe, Live cell membrane probe, orange/red
• UNSPSC Code: 41106514
• NACRES: NA.47

Properties

• mol wt: observed mol wt 1370.3 kDa
• Quality Level: 100
• packaging: vial of 50 μL
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell based assay: suitable; flow cytometry: suitable
• detection method: fluorometric
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The BioTracker^™ Membright-560 live cell dye is a “turn-on” fluorescence probe emitting in the orange to red spectrum for imaging of plasma membranes of live cells.

Probes specific for the plasma membrane are essential for demarcating cell boundaries. Membright dyes use a novel fluorogenic mechanism, operating in a self-quenched nanoparticle state. On contact and propagation within the cell membrane, fluorescence is turned on, producing a bright signal with low to no background. The Membright live cell membrane probes are compatible with two-photon imaging, superresolution microscopy, and can be used in live or fixed cells. Their robust photostability and lack of cytotoxicity make Membright probes optimal for protracted live cell imaging. The superior characteristics of these plasma membrane probes permit use in a diverse spectrum of fluorescence detection applications.

Spectral Properties
Fluorescence images obtained by λex = 560 nm and emission range of 567-750 nm.

Reference

Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614

Application

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.

Features and Benefits

Red/orange fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.

Physical form

Magenta solution

Storage and Stability

Store long term at -20°C, protected from light.

Other Notes

Live cell fluorescent imaging, flow cytometry

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup