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Sigma-Aldrich

Anti-5-Methylcytosine Mouse mAb (162 33 D3)

liquid, clone 162 33 D3, Calbiochem®

Synonym(s):

Anti-5-mc, Anti-5-MeCyd

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

162 33 D3, monoclonal

form

liquid

does not contain

preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
Flow Cytometry
Frozen Sections
Immunoblotting
Immunofluorescence
Paraffin Sections

Reliable
Generates reproducible results.
Clone 162 33 D3 has been used in over 50 publications.

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Immunogen

5-methylcytosine conjugated to ovalbumin

Application



ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
NIH3T3 cells

Other Notes

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gu Zhou et al.
BioTechniques, 62(4), 157-164 (2017-04-14)
Here, we present a DNA restriction enzyme-based, fluorescent cytosine extension assay (CEA) to improve normalization and technical variation among sample-to-sample measurements. The assay includes end-labeling of parallel methylation-sensitive and methylation-insensitive DNA restriction enzyme digests along with co-purification and subsequent co-measurement
Ikuo Matsuda et al.
Journal of clinical and experimental hematopathology : JCEH, 54(1), 67-73 (2014-06-20)
Lymphomas are malignant neoplasms composed of lymphoid cells at various developmental stages and lineages. Recent advances in comprehensive genomic analyses in acute myeloid leukemia have revealed prevalent mutations in regulators of epigenetic phenomena including global DNA methylation status. The examples
Dayanidhi Kumar et al.
PloS one, 8(7), e69927-e69927 (2013-08-08)
Cytogenetic studies have demonstrated that low levels of chronic radiation exposure can potentially increase the frequency of chromosomal aberrations and aneuploidy in somatic cells. Epidemiological studies have shown that health workers occupationally exposed to ionizing radiation bear an increased risk
Bo Xia et al.
International journal of toxicology, 35(3), 336-343 (2016-03-11)
Benzo[a]pyrene (B[a]P) exposure has been associated with the alteration in epigenetic marks that are involved in cancer development. Biotinidase (BTD) and holocarboxylase synthetase (HCS) are 2 major enzymes involved in maintaining the homeostasis of biotinylation, and the deregulation of this
Jun-Xue Jin et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(3), 1255-1266 (2017-03-08)
Hypoacetylation caused by aberrant epigenetic nuclear reprogramming results in low efficiency of mammalian somatic cell nuclear transfer (SCNT). Many epigenetic remodeling drugs have been used in attempts to improve in vitro development of porcine SCNT embryos. In this study, we

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