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Key Documents

ABS55

Sigma-Aldrich

Anti-InsP3R Antibody, Type 1

from rabbit, purified by affinity chromatography

Synonym(s):

inositol 1,4,5-triphosphate receptor, type 1, spinocerebellar ataxia 16, inositol 1,4,5-trisphosphate receptor type 1, Type 1 inositol 1,4,5-trisphosphate receptor, Type 1 InsP3 receptor, IP3 receptor, spinocerebellar ataxia 15, IP3 receptor isoform 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, pig

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), primate (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_001007236

UniProt accession no.

P29994

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITPR1(3708)

General description

The inositol (1,4,5)-trisphosphate receptor (InsP3R) regulates calcium release from storage within cells and plays an important role in neuronal function. InsP3R has been linked to Huntington’s disease. InsP3R has also been linked to the association between cAMP and Ca2+ signaling in regions of the brain, including the neostriatum.

Specificity

This antibody recognizes InsP3R at the cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat InsP3R.

Application

Immunohistochemistry Analysis: 1:1500 dilution from a previous lot detected InsP3R in purkinje cells at the junction of the granular and molecular layers of normal rat cerebellum tissue.

Immunoprecipitation Analysis: 10 µg of Anti-InsP3R, Type 1 immunoprecipitated InsP3R, Type 1 from rat brain microsomal prep RIPA lysate.
This Anti-InsP3R Antibody, Type 1 is validated for use in WB, IP, IH(P) for the detection of InsP3R.

Quality

Evaluated by Western Blot in rat brain microsomal tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected InsP3R on 10 µg of rat brain microsomal tissue lysate.

Target description

~300 kDa observed. UniProtKB/Swiss-Prot P29994 (ITPR1_RAT) entry describes 8 isoforms produced by alternative splicing: Isoform 1 at 313.264 kDa, Isoform 2 at 311.498 kDa, Isoform 3 at 313.136 kDa, Isoform 4 at 311.370 kDa, Isoform 5 at 311.210 kDa, Isoform 6 at 309.444 kDa, Isoform 7 at 308.674 kDa, and Isoform 8 at 306.909 kDa

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Melanie Vanessa Halbach et al.
Cerebellum (London, England), 16(1), 68-81 (2016-02-13)
Spinocerebellar ataxia type 2 (SCA2) is an autosomal dominantly inherited neurodegenerative disorder with preferential affection of Purkinje neurons, which are known as integrators of calcium currents. The expansion of a polyglutamine (polyQ) domain in the RNA-binding protein ataxin-2 (ATXN2) is
Cesar Cardenas et al.
Science signaling, 13(640) (2020-07-16)
Spontaneous Ca2+ signaling from the InsP3R intracellular Ca2+ release channel to mitochondria is essential for optimal oxidative phosphorylation (OXPHOS) and ATP production. In cells with defective OXPHOS, reductive carboxylation replaces oxidative metabolism to maintain amounts of reducing equivalents and metabolic

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