17-283
Rac1 Activation Assay Kit
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.
Synonym(s):
Activation Assay Kit, Rac1 Activity Kit, Rac1 Assay Kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Recommended Products
Quality Level
species reactivity
human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
affinity binding assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... RAC1(5879)
General description
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades. The RAC1 gene is located on the human chromosome at 7p22.1.
Application
Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.
Components
Anti-Rac1, clone 23A8 (Cat.# 05-389)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Quality
Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.
Linkage
Replaces: SGT425
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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The role of mechanical stretching in the activation and localization of adhesion proteins and related intracellular molecules.
Boccafoschi, F, et al.
Journal of Cellular Biochemistry, 112, 1403-1409 (2011)
The survival kinase Mirk/dyrk1B is activated through Rac1-MKK3 signaling.
Kideok Jin, Seunghwan Lim, Stephen E Mercer, Eileen Friedman
The Journal of Biological Chemistry null
rs12977 of RAC1 is associated with the progress of gastric cancer in Iranian population
Javani A, et al.
Gene Reports, 23, 101069-101069 (2021)
Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar et al.
Blood null
Michelle E Marchese et al.
Biochemistry, 51(41), 8235-8246 (2012-09-14)
Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for
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