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D-073

Supelco

5α-Dihydrotestosterone (DHT) solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Synonym(s):

5α-Dihydrotestosterone solution, 5-alpha-Dihydrotestosterone, DHT, Dihydrotestosterone, 5α−DHT solution

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About This Item

Empirical Formula (Hill Notation):
C19H30O2
CAS Number:
521-18-6
Molecular Weight:
290.44
MDL number:
MFCD00003667
UNSPSC Code:
41116107
PubChem Substance ID:
329798632
NACRES:
NA.24

grade

certified reference material

form

liquid

feature

Snap-N-Spike®/Snap-N-Shoot®

packaging

ampule of 1 mL

manufacturer/tradename

Cerilliant®

concentration

1.0 mg/mL in methanol

technique(s)

gas chromatography (GC): suitable
liquid chromatography (LC): suitable

application(s)

clinical testing
clinical testing

format

single component solution

storage temp.

−70°C

SMILES string

C[C@]12CC[C@H]3[C@@H](CC[C@H]4CC(=O)CC[C@]34C)[C@@H]1CC[C@@H]2O

InChI

1S/C19H30O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h12,14-17,21H,3-11H2,1-2H3/t12-,14-,15-,16-,17-,18-,19-/m0/s1

InChI key

NVKAWKQGWWIWPM-ABEVXSGRSA-N

General description

This Certified Spiking Solution® is suitable for GC/MS and LC/MS applications from clinical and diagnostic testing and endocrinology to sports testing.

5α-Dihydrotestosterone (DHT) is a steroid hormone and metabolite of the natural androgen, testosterone, formed by the action of NADPH-dependent enzyme 5α-reductase. It has been implicated in a broad range of diseases from male pattern baldness to benign prostatic hyperplasia and prostate cancer. As an anabolic steroid, DHT has also been used as a performance-enhancing drug to promote muscle growth.

Application

The Certified Reference Material (CRM) in solution can also be used as follows:
  • Simultaneous analysis of testosterone and 5α-dihydrotestosterone using a benchtop gas chromatograph-mass spectrophotometer (GC-MS) in human serum samples
  • Liquid chromatography-electrospray ionization-tandem mass spectrometric (LC/ESI-MS/MS) determination of testosterone and 5α-dihydrotestosterone following their proton affinitive derivatization using picolinic acid and its analogs
  • Determination of testosterone and 5α-dihydrotestosterone in plasma and testis samples of fathead minnow by a gas chromatography-tandem mass spectrometric (GC-MS/MS) method
  • Multi-residue screening of epitestosterone, testosterone, 5α-dihydrotestosterone, dehydroepiandrosterone, androsterone, and etiocholanolone in urine samples by high-resolution full scan-electrospray ionization-liquid chromatography (HR-FS-ESI-LC) combined with quadrupole orbitrap mass spectrometer (Q/Orbitrap MS) following requirements of World Antidoping Agency (WADA)
  • Quantitative analysis of nine hydroxy-androgens and their conjugates by a stable isotope dilution liquid chromatography-electrospray ionization-tandem mass spectrometry (SID-LC-ESI-MS/MS) based method following their derivatization using picolinic acid

Features and Benefits

  • Fully characterized under ISO/IEC 17025 and ISO 17034 accreditation
  • Accompanied with a comprehensive Certificate of Analysis (CoA) with data on stability, homogeneity, accuracy of concentration, uncertainty, and traceability
  • Rigorously tested through real-time stability studies to ensure accuracy and shelf life
  • Gravimetrically prepared using qualified precision balances to ensure minimal uncertainty
  • Flame sealed under argon into ampoules for long-term shelf life
  • Offered in a convenient, DEA-exempt format to improve laboratory efficiency

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

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E-058

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H-059

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N-050

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T-037

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T-046

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T-070

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T-510

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Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

49.5 °F - closed cup

Flash Point(C)

9.7 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Julia Johansson et al.
PloS one, 8(1), e54357-e54357 (2013-01-26)
In rats with dihydrotestosterone (DHT)-induced polycystic ovary syndrome (PCOS), repeated low-frequency electrical stimulation of acupuncture needles restores whole-body insulin sensitivity measured by euglycemic hyperinsulinemic clamp. We hypothesized that electrical stimulation causing muscle contractions and manual stimulation causing needle sensation have
Angel On Kei Chan et al.
Clinical chemistry, 59(5), 798-806 (2013-03-21)
5α-Reductase 2 deficiency (5ARD) is a known cause of 46,XY disorders of sex development (DSD). Traditionally, the diagnosis relies on dihydrotestosterone (DHT) measurement, but the results are often equivocal, potentially leading to misdiagnosis. We reviewed alternative approaches for diagnosis of
Changmeng Cai et al.
The Journal of clinical investigation, 123(3), 1109-1122 (2013-02-22)
Fusion of the androgen receptor-regulated (AR-regulated) TMPRSS2 gene with ERG in prostate cancer (PCa) causes androgen-stimulated overexpression of ERG, an ETS transcription factor, but critical downstream effectors of ERG-mediating PCa development remain to be established. Expression of the SOX9 transcription
Paula M Marchetti et al.
Annals of clinical biochemistry, 50(Pt 2), 95-107 (2013-02-23)
Dihydrotestosterone (DHT) is the most potent natural androgen in humans. There has been an increasing interest in this androgen and its role in the development of primary and secondary sexual characteristics as well as its potential roles in diseases ranging
Paulo A Saldanha et al.
Clinical and experimental pharmacology & physiology, 40(3), 181-189 (2013-01-03)
The aim of the present study was to determine the effects of androgens in the regulation of human umbilical artery (HUA) contractility. The short-term effects of testosterone on the tone of the HUA were investigated, as were the long-term effects
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