Validated Cell Types for the Scepter™ 3.0 Cell Counter
The Scepter™ counter has been shown to be accurate over a range of cell types and sizes. See below for a list of mammalian and other cell types that have been validated for Scepter™ counting.
Measured
• Recommended based on size
• Customer Validated
• MilliporeSigma Scepter™ 2.0 Validated
• MilliporeSigma Scepter™ 3.0 Validated
1 histograms may be cut-off at high concentrations, dilution may be needed
Cell Type | size (μm) | 40 μm sensor | 60 μm sensor |
---|---|---|---|
2102 Ep | 15-19 | • | |
454 beads | 28 | • | |
A10 | 22 - 24 | • | |
A172 | 15 | • | |
A253 | 14-18 | • | |
A375 | 16 | • | |
A431 | 15-17 | • | |
A549 | 11-15 | • | |
Algae (various) | 7-9 | • | • |
B35 | 13-16 | • | • |
B Cells | 6-11 | • | |
C2C12 | 12 | • | • |
C305 | 12-14 | • | • |
C6 | 12-13 | • | |
CA46 | 10-12 | • | • |
Caco-2 | 17 | •• | |
CHO | 13-17 | •• | |
COS-1 | 12 | • | |
Cos-7 | 15 | • | |
D283 | 12 | • | |
Daudi | 10-12 | • | • |
DU-145 | 15-17 | • | |
EL4 (Blood Murine Lymphoblast) | 12-14 | • | • |
Epithelia | 14-15 | • | |
Epithelia MDA-MB-231 | 16-17 | • | • |
HCC827 (Human lung adenocarcinoma) | 18 | • | |
HCT-116 | 10 | • | • |
HEK293 | 11-16 | •• | |
HeLa | 12-15 | • | •• |
HepG2 | 12-17 | •1 | •• |
HFF | 18-20 | • | |
Hs27 | 14 | • | • |
HT-1080 (fibrosarcoma) | 14-16 | •• | |
HT-29 | 11 | • | |
HUH7 (Hepatoma) | not available | ||
Human ES Cells | 9-12 | • | |
HUVEC | 14-15 | • | |
Hybridoma | 13-14 | • | |
IMR-32 | 12-14 | • | • |
IMR-90 | 15 | • | • |
iPSCs | 16-17 | •1 | • |
Jurkat | 12-13 | • | •• |
K562 | 22 | • | |
KB | 14 | • | |
KG-1 | 10-13 | • | • |
L6 | 14-16 | • | |
LNCaP | 15-16 | • | |
Luminex®beads | 5-6 | • | |
MCF7 | 15-21 | •• | |
MDCK | 13-15 | • | •• |
Measured
• Recommended based on size
• Customer Validated
• MilliporeSigma Scepter™ 2.0 Validated
• MilliporeSigma Scepter™ 3.0 Validated
1 histograms may be cut-off at high concentrations, dilution may be needed
Cell Type | size (μm) | 40 μm sensor | 60 μm sensor |
---|---|---|---|
Meg-01 | 16-17 | • | |
MG-63 | 15-17 | • | |
Mouse ES Cell | 5-13 | • | • |
MN9D (Mouse Dopaminergic Neuronal) | 15 | • | |
MSc (Mesenchymal Stem) | 15-16 | ••1 | • |
MRC-5 | 18 | • | |
NCI-H146 | 10-13 | • | |
NIH 3T3 | 15 | • | •• |
NTERA2, clone D1 | 13 | • | • |
OK | 17-18 | • | |
PBMCs | 7-12 | • | |
PC12 | 9-15 | • | • |
Primary Astrocytes | 7 | • | |
Primary Neuronal Cell | Varies | • | |
Raji | 12-15 | • | • |
Ramos | 11-12 | • | • |
Rat Dorsal Root Ganglion Cells | 20-30 | • | |
Rat Whole Blood | 4.6 | •• | |
Red Blood Cells | 5-7 | • | |
Rat Neural Stem Cell (RNSC) | 11-13 | • | |
RAW 264.7 Cell Line murine macrophage from blood | 11-13 | • | • |
RBL | 11-13 | • | • |
RIN-mF5 | 13-14 | • | |
S462 (Human Peripheral Nerve Sheath Tumor) | 16 | • | |
SF9 | 13 | • | |
SH-SY5Y | 12 | • | |
Sk-Br-3 | 15-20 | • | |
SK-MEL-28 | 17-19 | • | |
SK-N-MC | 14-15 | • | |
SK-N-SH | 14-15 | • | |
Splenocytes | 7-9 | • | |
SW-480 | 15 | • | |
SW-620 | 13-14 | • | • |
T84 | 14-18 | • | |
T98G | 17 | • | |
T cells: naïve | 5-7 | • | |
T cells: CD3+ | 11-12 | • | • |
THP 1 (Human blood monocyte) | 12-13 | • | • |
TF-1 | 13-14 | • | • |
U251 | 16-20 | • | |
U2OS | 16-19 | • | |
U266 | 12 | • | |
U87 (Human Glioblastoma) | 12-14 | • | |
U937 | 11-13 | • | • |
WI-38 | 12-15 | • | |
Y79 | 13-14 | • | |
Yeast- Pichia Pastoris | 5 | • | |
Yeast - S. cerevisiae | 6 | • |
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