LC/MS/MS of Phospholipids on Ascentis® C18
Materiales
patrón
Referencia del producto
Descripción
Precios
L-α-Phosphatidylinositol ammonium salt solution from bovine liver
10 mg/mL in chloroform, ≥98% (TLC)CONDITIONS
column
Ascentis C18, 15 cm x 2.1 mm I.D., 3 μm particles (581365-U)
mobile phase
10 mM ammonium formate in [A] water; [B] methylene chloride; [C] methanol; (2:3:5, A:B:C)
flow rate
0.2 mL/min
pressure
1270 psi (87 bar)
column temp.
25 °C
detector
MS, ESI(+), MRM as indicated on the figure
injection
2 μL
sample
300 mg/L in mobile phase
Descripción
Nota de análisis
An LC/MS/MS is shown here for the separation and detection of phospholipids on an Ascentis C18 reversed-phase column with high separation efficiency and excellent peak shape.
Información legal
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany