HPLC analysis of Methyl Paraben and Organic Acids on Ascentis^® Express RP-Amide

column

Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (50774-U)

mobile phase

[A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)

flow rate

1.3 mL/min

pressure

1870 psi (129 bar)

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

[A] water; [B] acetonitrile; (50:50, A:B)