HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile; (85:15, A:B)

flow rate

1.2 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

4 μL

sample

200 ng/mL in 25:75, water: acetonitrile