- miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.
miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.
The Journal of experimental biology (2017-06-04)
Mary Latimer, Nathalie Sabin, Aurélie Le Cam, Iban Seiliez, Peggy Biga, Jean-Charles Gabillard
PMID28576820
RESUMEN
In fish, data on microRNAs (miRNAs) involved in myogenesis are scarce. In order to identify miRNAs involved in satellite cell differentiation, we used a methionine depletion/replenishment protocol to synchronize myogenic cell differentiation. Our results validated that methionine removal (72 h) from the medium strongly decreased
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Reactivo TRI®, For processing tissues, cells cultured in monolayer or cell pellets
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Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
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DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture
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Nutrient Mixture F-10 Ham, With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture