TBS 10X Stock Solution for Western Blots
Tris-buffered saline (TBS) is an excellent wash buffer for many types of immunoassays. To make 1 L of 10X TBS stock solution, dissolve 24 g Tris and 88 g NaCl in 900 mL of water and then adjust the pH to 7.6 and final volume to 1 L.
This calculator enables the preparation of a 10X TBS wash buffer stock solution, whether you are preparing enough for a single experiment or for the entire lab. Input your desired volume and click the CALCULATE button. The right side of the table will populate with the correct amounts of each reagent needed for your desired total final volume.
Solution concentrations:
- Tris: 200 mM
- NaCl: 1500 mM
| 1 L Recipe | Desired Volume: mL |
| 24 g of Tris Base (FW 121.14) (93362) | g of Tris Base |
| 88 g of NaCl (FW 58.44) (71376) | g of NaCl |
| Dissolve in 900 mL Milli-Q® water. | Adjust pH to 7.6 with 12 N HCl (H1758). |
| Adjust pH to 7.6 with 12 N HCl (H1758). | Adjust final volume to mL with Milli-Q® Water |
| Adjust final volume to 1 L with Milli-Q® Water | Adjust pH to 7.6 with 12 N HCl before adjusting to final volume with Milli-Q® water. |
| Prepared TBS is stable at 4 °C for 3 months. |
OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer
- 10X Phosphate-Buffered Saline (PBS)
- Stock Components for Western Blot Stripping Solutions
Wash & Blocking Buffers
- 1X Tris-Buffered Saline (TBS) Working Solution
- 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
- 1X Phosphate-Buffered Saline (PBS) Recipe Calculator
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Non-Fat Dry Milk (NFDM) Blocking Buffer
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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