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565303-U

Supelco

Ascentis® RP-Amide (5 µm) HPLC Columns

L × I.D. 5 cm × 2.1 mm, HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm

Materiales

stainless steel column

Agency

suitable for USP L60

Línea del producto

Ascentis®

Características

endcapped

fabricante / nombre comercial

Ascentis®

envase

1 ea of

Extensión del etiquetado

19.5% Carbon loading

Parámetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnicas

HPLC: suitable
LC/MS: suitable

L × D.I.

5 cm × 2.1 mm

superficie

450 m2/g

cobertura de la superficie

2.7 μmol/m2

impurezas

<5 ppm metals

matriz

fully porous particle
silica gel high purity, spherical

grupo activo de la matriz

amide, alkyl phase

tamaño de partícula

5 μm

tamaño de poro

100 Å

operating pH range

2-8

aplicaciones

food and beverages

técnica de separación

reversed phase

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Descripción general

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Características y beneficios

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificados de análisis (COA)

Lot/Batch Number
289211-02
289211-01
289167-02
289167-01
289140-02

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

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