ZRB2520

Anti-PKD/PKCμ Antibody, clone 1C11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: EC:2.7.11.13, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1C11, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 101.70 kDa; observed mol wt ~115 kDa
• purificado por: using Protein A
• reactividad de especies: human
• envase: antibody small pack of 25
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgGκ
• secuencia del epítopo: C-terminal
• Nº de acceso Protein ID: NP_002733.2
• Nº de acceso UniProt: Q15139
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... PRKD1(5587)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1C11is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects PKD/PKC . It targets an epitope within 18 amino acids from the C-terminal region of the protein.

Inmunógeno

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human PKD/PKC .

Aplicación

Quality Control Testing

Evaluated by Western Blotting in lysate from LNCaP cells.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected PKD/PKC in lysate from LNCaP cells.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected PKD/PKC in lysate from HuH-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound with PKD/PKC peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PKD/PKC in LNCaP cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PKD/PKC in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Serine/threonine-protein kinase D1 (UniProt: Q15139; also known as Protein kinase C mu type, EC:2.7.11.13, Protein kinase D, nPKC-D1, nPKC-mu) is encoded by the PRKD1 (also known as PKD, PKD1, PRKCM ) gene (Gene ID: 5587) in human. The PRKD1 gene promoter has a CpG island covering 1.2 kb and It includes, the transcription start site and the exon 1. The Serine/threonine-protein kinase D1 protein structure features a conserved catalytic Protein Kinase domain at the C-terminus and, an N-terminal regulatory domain that includes a diacylglycerol (DAG)/phorbol ester-binding C1 domain and a pleckstrin homology (PH) domain. Typically, the protein is found in the cytoplasm; however, upon activation, it can translocate to the plasma membrane, golgi appartaus or mitochondria. This translocation is crucial for targeting specific substrates and implies a spatial regulation of its kinase activity. PRKD1 is ubiquitously expressed in various human tissues, with significant expression in the heart, skeletal muscle, and brain. It is also expressed in cells of the normal mammary gland. The protein is involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, vesicle transport, cell survival, and regulation of cell shape and adhesion. In mammary glands, it preserves the epithelial phenotype by inhibiting epithelial-to-mesenchymal transition. Overexpression and dysregulation of PRKD1 are observed in several cancers, including prostate, gastric, and pancreatic cancers, suggesting a role in tumor progression and metastasis. Moreover, PRKD1 has been linked to cardiovascular diseases and neurodegenerative disorders, where its signaling pathways impact cell survival and function. There is also emerging evidence suggesting PRKD1′s role in immune system modulation, potentially impacting autoimmune diseases and inflammation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref; Zhang W., et al., J Biol Chem 2005; 280(19): 19036-19044; Hurd C., et al., Oncogene 2002; 21(14): 2154-60.

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable