ZRB2396

Anti-MST1/STK4 Antibody, clone 1L17-L2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: EC: 2.7.11.1, Mammalian STE20-like protein kinase 1, STE20-like kinase MST1, Serine/threonine-protein kinase 4, Serine/threonine-protein kinase Krs-2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1L17-L2, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• purificado por: using Protein A
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse, rat
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: N-terminal
• Nº de acceso Protein ID: NP_006273
• Nº de acceso UniProt: Q13043
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... STK4(6789)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1L17-L2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Serine/threonine-protein kinase 4 (MST1/STK4). It targets an epitope within 16 amino acids from the N-terminal region. Detects the intact and cleaved forms.

Inmunógeno

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Serine/threonine-protein kinase 4 (MST1/STK4).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MST1/STK4 in Ramos cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MST1/STK4 in Caco2 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound MST1/STK4 peptide with a KD of 1.8x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MST1/STK4 in Human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MST1/STK4 in Ramos cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Serine/threonine-protein kinase 4 (UniProt: Q13043; also known as EC: 2.7.11.1, Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2, STK4) is encoded by the STK4 (also known as KRS2, MST1) gene (Gene ID: 6789) in human. STK4 is a homodimeric, stress-activated, pro-apoptotic kinase that enters the nucleus following its caspase cleavage and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is ubiquitously expressed with higher expression observed in prostate cancer and its levels increase with the progression to malignant state. Its protein kinase domain is localized in residues 30-281. It is activated by caspase-cleavage between amino acids 326-327 and 349-350. The caspase-cleaved form cycles between the nucleus and cytoplasm. Its full activation requires homodimerization and autophosphorylation at threonine 183. Its activity is inhibited by the C-terminal non-catalytic region. STK4 is a KEY^® component of the Hippo signaling pathway, which plays a pivotal role in organ size control and acts as a tumor suppressor by restricting cell proliferation and promoting apoptosis. During apoptosis, it phosphorylates serine 14 of histone H2B and phosphorylates FOXO3 upon oxidative stress that results in its NuCLEAR^™ translocation and initiation of cell death. Its phosphorylation at threonine 387 by AKT1, LEADS^™ to inhibition of its kinase activity, NuCLEAR^™ translocation and autophosphorylation at threonine 183. It also reduces its cleavage by caspases and its ability to phosphorylate FOXO3. Two isoforms of STK4 have been described that are produced by alternative splicing. Mutations in STK4 gene have been linked to T cell immunodeficiency resulting from progressive loss of naive T-cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Guennoun, A., et al. (2021). J. Clin. Immunol. 41(8); 1839-1852; Praskova, M., et al. (2004). Biochem. J. 381(Pt2); 453-462; Lee, KK., et al. (2001). J. Biol Chem. 276(22); 19276-19285).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

Affinity is a trademark of Mine Safety Appliances Co.

KEY is a registered trademark of Hanna Instruments

LEADS is a trademark of Compugen

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable