ZRB2273

Anti-POLQ Antibody, clone 2F5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Anti-POLQ Antibody, clone 2F5 ZooMAb^® Rabbit Monoclonal, DNA polymerase eta, DNA polymerase theta, EC:2.7.7.7
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2F5, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 290 kDa; observed mol wt ~200 kDa
• purificado por: using Protein A
• reactividad de especies: human
• envase: antibody small pack of 25
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: N-terminal half
• Nº de acceso Protein ID: NP_955452
• Nº de acceso UniProt: O75417
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... POLQ(10721)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 2F5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects DNA polymerase theta (POLQ). It targets an epitope within 23 amino acids from the N-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human DNA polymerase theta (POLQ).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected POLQ in U2OS cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected POLQ in Raji cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected POLQ in Human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected POLQ in U2OS cells.

Affinity Binding Assay: A representative lot of this antibody bound POLQ peptide with a KD of 1.8 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

DNA polymerase theta (UniProt: O75417; also known as EC:2.7.7.7, DNA polymerase eta, POLQ) is encoded by the POLQ (also known as POLH) gene (Gene ID: 10721) in human. POLQ is a homomultimeric enzyme that is minimally expressed in normal tissues but is overexpressed in tumors. Its expression is observed in testis tissue. It contains a helicase-like domain at its N-terminus and a polymerase domain at its C-terminus. They are separated by a large unstructured central domain. It promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery that is triggered in response to double-strand breaks in DNA. It acts by binding directly the 2 ends of resected double-strand breaks, allowing micro homologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. It requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. POL

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable