ZRB2132

Anti-Ago 1 Antibody, clone 1L3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C1;eIF2C1;Putative RNA-binding protein Q99
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1L3, recombinant monoclonal
• descripción: 1L3 Clone
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 97.21 kDa; observed mol wt ~105 kDa
• purificado por: using Protein A
• reactividad de especies: monkey, human, mouse
• reactividad de especies (predicha por homología): bovine, sheep, feline
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: Internal
• Nº de acceso Protein ID: NP_001304051
• Nº de acceso UniProt: Q9UL18
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... AGO1(26523)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1L3 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Protein argonaute-1 (Ago 1). It targets an epitope within 21 amino acids from the internal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Protein argonaute-1 (Ago 1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in K562 and COS-7 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in human placenta tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound Ago1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.

Descripción de destino

Protein argonaute-1 (UniProt: Q9UL18; also known as Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99) I encoded by the AGO1 (also known as EIF2C1) gene (Gene ID: 26523) in human. The highly conserved Argonaute proteins play a critical role in the regulation of gene expression and orchestrate the establishment and the maintenance of cell identity throughout the entire life cycle. In humans, four functional Argonaute proteins (Ago 1-4) have been described that exhibit high structure similarity, but also have a few non-conserved amino acids in their functional domains. In contrast to Ago 2, which has two N-terminal motifs that are required for its full catalytic activity, Ago1 contains only one N-terminal motif and Ago 3 and 4 lack these motifs and are catalytically inactive. About 80% of Ago1 clusters are associated with cell-type-specific transcriptional enhancers. Ago1 is required for RNA-mediated gene silencing. It binds to short RNAs such as miRNA or siRNA and represses the translation of mRNAs which are complementary to them. Its PIWI domain is localized in amino acids 515 to 816. Ago1 lacks RNA cleavage activity due to the absence of the conserved histidine at position 805. Ago1 is shown to be enriched around transcription start sites and binds preferentially to active transcriptional enhancers. Its association with enhancers is mediated by RNAs that are transcribed from these enhancers. However, the interaction of Ago1 with enhancers does not appear to regulate transcription of the neighboring genes. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Müller, M., et al. (2020). Front. Cell Dev. Biol. 7; 360; Allo, M., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(44); 15622-15629).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable