ZRB2078

Anti-ASH2L Antibody, clone 2B4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Set1/Ash2 histone methyltransferase complex subunit ASH2;ASH2-like protein
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2B4, recombinant monoclonal
• descripción: 2B4 Clone
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 68.72 kDa; observed mol wt ~78 kDa
• purificado por: using Protein A
• reactividad de especies: human
• reactividad de especies (predicha por homología): feline, mouse, bovine, rat, monkey, canine, porcine
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_004665
• Nº de acceso UniProt: Q9UBL3
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ASH2L(9070)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 2B4 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects ASH2. It targets an epitope within 19 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Set1/Ash2 histone methyltransferase complex subunit ASH2.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ASH2L in HL-60 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ASH2L in Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ASH2L in human colon tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound ASH2L peptide with a KD of 1.2 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Set1/Ash2 histone methyltransferase complex subunit ASH2 (UniProt: Q9UBL3; also known as ASH2-like protein) is encoded by the ASH2L (also known as ASH2L1) gene (Gene ID: 9070) in human. The Absent, Small, Homeotic discs 2-Like (ASH2L) is a transcriptional regulator that is a component of the Set1/Ash2 histone methyltransferase (HMT) complex composed of ASH2L, RBBP5, WDR5, HDPY-30, one of seven SET/MLL proteins (SETA, SETB, MLL1 to MLL5), and additional MLL complex-specific non-core proteins. This complex specifically methylates lysine 4 of histone H3. In association with RBBP5 and WDR5, it stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B. ASH2L is ubiquitously expressed in humans with the highest level of expression detected in the fetal liver and testis. Its expression is also observed in adult heart and testis. It plays a crucial role in mammalian development. Mice lacking ASH2L die prior to embryonic implantation. It can undergo monomethylation and dimethylation on arginine residues in the C-terminus with arginine 296 being the major site. However, methylation is not required for its nuclear localization and MLL complex integrity. Overexpression of ASH2L is reported to enhance p53-dependent apoptosis in response to chemotherapy. ASH2L contains a DNA-binding Forkhead-like DNA binding domain (aa 67-177) and an atypical PHD-type domain (aa 1-66) at its N-terminal region. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mungamuri, SK., et al. (2015). Oncogene. 34; 2461-2470; Butler, JS., et al. (2011). J. Biol. Chem. 286(14); 12234-12244; Stroller, JZ., et al. (2010). Exp. Biol. Med. 235(5); 569-576).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable