ZRB1524

Anti-DHX37 Antibody, clone 3I16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 3I16, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 129.55 kDa; observed mol wt ~150 kDa
• purificado por: using Protein A
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: N-terminal
• Nº de acceso Protein ID: NP_116045
• Nº de acceso UniProt: Q8IY37
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 3I16 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects DEAH box protein 37 (DHX37). It targets an epitope within 24 amino acids from the N-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human DEAH box protein 37 (DHX37).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DHX37 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DHX37 in U2OS and Jurkat cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound DHX37 with a KD of 2.3 x 10-10 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DHX37 in human placenta and human skin tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DHX37 in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-DHX37, clone 3I16 ZooMAb^®, Cat. No. ZRB1524, is a recombinant Rabbit monoclonal antibody that targets DHX37 and is tested for use in is used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Descripción de destino

Probable ATP-dependent RNA helicase DHX37 (UniProt: Q8IY37; also known as EC:3.6.4.13, DEAH box protein 37) is encoded by the DHX37 (also known as DDX37, KIAA1517) gene (Gene ID: 57647) in human. DHX37, a member of the DEAD box helicase family, is an ATP-binding RNA helicase that plays a role in maturation of the small ribosomal subunit in ribosome biogenesis. It is required for the release of the U3 snoRNP from pre-ribosomal particles. Lack of DHX37 is reported to trigger a surveillance pathway that leads to degradation of pre-ribosomal particles and a depletion of DHX37 is shown to inhibit the conversion of the 21S pre-rRNA to 18SE. Its expression has been reported in fallopian tube, ovary, uterus, and testis and is also detected in the brain. Its DEAH box motif is localized to amino acids 372-375. It plays a role in early testis development and its pathogenic variants are reported to cause 46,XY gonadal dysgenesis and lead to testicular regression syndrome. Mutations in DHX37 gene are also reported to cause neurodevelopmental disorders with brain anomalies that are characterized by severe developmental delays, cortical volume loss and cerebellar hypoplasia. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: McElreavey, K., et al. (2020). Genet. Med. 22(1); 150-159; Choudhury, P., et al. (2019). RNA Biol. 16(1); 54-68).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable