ZRB1066

Anti-PNPT1 Antibody, clone 2D23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: 3′-5′ RNA exonuclease OLD35, EC:2.7.7.8, PNPase 1, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, Polyribonucleotide nucleotidyltransferase 1 mitochondrial
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• clon: 2D23, recombinant monoclonal; recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 85.95 kDa; observed mol wt ~80 kDa
• reactividad de especies: mouse, rat, human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso UniProt: A8TCS8
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone 2D23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects PNPase 1 (PNPT1). It targets an epitope within 20 amino acids from the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human PNPase 1 (PNPT1).

Aplicación

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PNPT1 in HeLa cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected PNPT1 in Jurkat, NIH3T3, and C6 cell lysates.Affinity Binding Assay: A representative lot of this antibody bound PNPT1 peptide with a KD of 2.5 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PNPT1 in human stomach tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PNPT1, clone 2D23 ZooMAb, Cat. No. ZRB1066, is a recombinant Rabbit monoclonal antibody that specifically targets PNPT1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin) and, Western Blotting.

Descripción de destino

Polyribonucleotide nucleotidyltransferase 1, mitochondrial (UniProt: A8TCS8; also known as EC:2.7.7.8, 3′-5′ RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like protein, PNPT1) is encoded by the PNPT1 (also known as PNPASE) gene (Gene ID: 87178) in human. PNPT1 is a peripheral homotrimer membrane protein that serves as a RNA-binding protein that is implicated in several RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3′-to-5′ direction. It is synthesized with a transit peptide (aa 1-45) and is localized to mitochondrial intermembrane region. It is a component of the mitochondrial degradosome (mtEXO) complex with exoribonuclease activity that degrades 3′ overhang double-stranded RNA with a 3′-to-5′ directionality in an ATP-dependent manner. It is reported to regulate the expression of the electron transport chain components at the mRNA and protein levels. PNPT1 is shown to be essential for correct processing and polyadenylation of mitochondrial mRNAs and plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components into the mitochondrial matrix. It is also reported to participate in RNA cell surveillance by cleaning up oxidized RNAs. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable