ZRB1007

Anti-Ki67 Antibody, clone 1O15, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Antigen KI-67, Antigen Ki67, Antigen identified by monoclonal antibody Ki-67, Proliferation marker protein Ki-67
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit (recombinant)
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1O15, recombinant monoclonal; monoclonal
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 358.69 kDa; observed mol wt ~350 kDa
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: immunocytochemistry: suitable using 1:100; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400; western blot: suitable using 1:1,000
• isotipo: IgG
• Nº de acceso UniProt: MKI67
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MKI67(4288)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb^® here.

Especificidad

Clone 1O15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Proliferation marker protein Ki-67. It targets an epitope with in 14 amino acids from the C-terminal half.

Inmunógeno

KLH conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Proliferation marker protein Ki-67.

Aplicación

Anti-Ki67, clone 1O15 ZooMAb, Cat. No ZRB1007, is a recombinant Rabbit Monoclonal Antibody that specifically targets Proliferation marker protein Ki-67 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ki67 in HUVEC, HeLa, and A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Ki67 in human tonsil and human skin tissue sections.

Descripción de destino

Proliferation marker protein Ki-67 (UniProt: P46013; also known as Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67) is encoded by the MKI67 gene (Gene ID: 4288) in human. Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle. Its expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen is undetectable. Ki67 is hyperphosphorylated in mitosis. Ki67 is required to maintain individual mitotic chromosomes and it disperses in the cytoplasm following nuclear envelope disassembly. It is shown to associate with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. It prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier. Two isoforms of Ki67 have been described that are produced by alternative splicing. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable