ZMS1180

Anti-LAG3 Antibody, clone 4-10-C9 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Anti-LAG3 Antibody, clone 4-10-C9 ZooMAb^® Mouse Monoclonal, Activation-induced cytidine deaminase-linked autoimmunity protein, Aida, CD223, LAG-3, Lymphocyte activation gene 3 protein
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4-10-C9, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 56.98 kDa; observed mol wt ~80 kDa
• purificado por: using protein G
• reactividad de especies: mouse
• envase: antibody small pack of 25
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• secuencia del epítopo: Extracellular domain
• Nº de acceso Protein ID: NP_032505
• Nº de acceso UniProt: Q61790
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: mouse ... Lag3(16768)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 4-10-C9 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects LAG3. It targets an epitope within D3/D4 region from the extracellular domain.

Inmunógeno

Murine LAG-3-expressing mouse T-cell hybridoma.

Aplicación

Quality Control Testing

Evaluated by Western Blotting with recombinant fragment corresponding to the extracellular domain of Mouse LAG3.

Western Blotting (WB) Analysis: A1:1,000 dilution of this antibody detected recombinant fragment corresponding to the extracellular domain of Mouse LAG3 fused to Fc portion of mouse IgG2a.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LAG3 in Mouse splenocytes treated with Concanavalin A (2 g/mL; 3 days).

Affinity Binding Assay:: A representative lot of this antibody bound recombinant fragment corresponding to the extracellular domain of Mouse LAG3 with a KD of 2.8 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Lymphocyte activation gene 3 protein (UniProt: Q61790; LAG-3, Activation-induced cytidine deaminase-linked autoimmunity protein, Aida, CD223) is encoded by the Lag3 gene (Gene ID: 16768) in murine species. LAG3 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-442), a transmembrane domain (aa 443-463), and a cytoplasmic domain (aa 464-521). It contains three Ig-like C2-type domains and one Ig-like V-type domain. LAG3 is primarily expressed in activated CD4+ and CD8+ T-cells. Its expression is also observed in a subset of regulatory T cells. It serves as an inhibitory receptor on antigen activated T-cells and deliver inhibitory signals upon binding to ligands, such as Fibrinogen-like protein 1 (FGL1). It can negatively regulate the proliferation, activation, effector function and homeostasis of both CD8+ and CD4+ T-cells. Following

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitución

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1