ZMS1013

Anti-MAP2 Antibody, clone AP20 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Microtubule-associated protein 2A
• Código UNSPSC: 12352203
• NACRES: NA.74

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: AP20, monoclonal; recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 217 kDa; observed mol wt ~260 kDa
• reactividad de especies: rat, mouse, human
• reactividad de especies (predicha por homología): Xenopus, bovine, quail, chicken
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso UniProt: F1MEW3
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: chicken ... Map2(424001) ; frog ... Map2(780043) ; human ... MAP2(4133) ; mouse ... Map2(17756) , Map2(281294) ; rat ... Map2(25595)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone AP20 is a is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects MAP2.

Inmunógeno

Full-length purified microtubule protein (MAP2) from bovine brain.

Aplicación

Anti-MAP2, clone AP20 ZooMAb, Cat. No. ZMS1013, is a recombinant Mouse monoclonal antibody that specifically targets MAP2 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAP2 in mouse and rat brain tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAP2 in SH-SY5Y cell line and E18 cortical neurons.

Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected MAP2 in mouse brain and rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descripción de destino

Microtubule-associated protein (UniProt: F1MEW3; also known as MAP2) is encoded by the MAP2 gene (Gene ID: 281294) in bovine. MAP2 is a neuron-specific protein that stabilizes microtubules in the dendrites of postmitotic neurons. Its major function is to stabilize neuronal shape by promoting microtubule synthesis and cross-linking with other components of the cytoskeleton. Phosphorylation of MAP2 controls its association with the cytoskeleton and it is developmentally regulated. MAP2 can undergo phosphorylation by GSK-3 and p44/42 MAP kinase. Phosphorylation of MAP2 at threonine 1620 and 1623 by GSK-3 are reported to inhibits MAP2 association with microtubules and affect microtubule stability. MAP2 consists of at least three isoforms produced from a single gene: high molecular mass MAP2a and MAP2b, and low molecular mass MAP2c that is also known as the juvenile MAP2c, which is expressed only during early development and exists mainly in axons. MAP2a appears to replace MAP2c during growth and MAP2b is expressed throughout life. MAP2 can also serve as a marker of synaptic plasticity and its levels are observed to increase after traumatic brain injury. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Soltani, MH., et al. (2005). Am. J. Pathol. 166(6); 1841-1850).

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1