X0629

Anti-XRCC1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-X-ray repair complementing defective repair in Chinese hamster cells 1
• Número MDL: MFCD01865396
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 85 kDa
• reactividad de especies: human
• concentración: ~1 mg/mL
• técnicas: indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using A431 cell lysates
• Nº de acceso UniProt: P18887
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... XRCC1(7515) ; mouse ... Xrcc1(22594) ; rat ... Xrcc1(84495)

Descripción

Descripción general

X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene.

Inmunógeno

synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat.

Aplicación

Anti-XRCC1 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• indirect immunofluorescence and telomere FISH (TEL-FISH)
• protein detection
• western analysis

Acciones bioquímicas o fisiológicas

X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)