W4387

Wee1, active from rat

N-terminal GST-tagged, solution

• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD03459055
• Biological source: rat
• Recombinant: expressed in E. coli

Propiedades

• biological source: rat
• Quality Segment: 200
• recombinant: expressed in E. coli
• form: solution
• mol wt: ~110 kDa
• UniProt accession no.: Q63802
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: rat ... Wee1(308937)

Descripción

Application

Wee1, active from rat may be used to study apoptotic signaling.

Biochem/physiol Actions

Protein inhibitor of Cdk2 kinase

Wee1 is a protein tyrosine kinase that phosphorylates Cdc2 on Tyr15 and hence inactivates the CDK1 and CDK2, which facilitates in the negative regulation of entry of cells into mitosis.[1] Increased level of wee1 stimulates Human Immunodeficiency Virus type 1 (HIV-1) Vpr induced cell cycle arrest at G2/M transition followed by apoptosis.[2]

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% Glycerol

Other Notes

One unit of Wee 1 is defined as the amount of Wee 1 which inhibits 90% of the activity of 10 units of Cdk2

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)