U7757
Anti-UCP-3 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Anti-SLC25A9
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About This Item
Productos recomendados
origen biológico
rabbit
conjugado
unconjugated
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
formulario
buffered aqueous solution
mol peso
antigen ~34 kDa
reactividad de especies
human, rat, mouse
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using protease-digested sections of human and mouse skeletal muscle.
western blot: 1:1,000 using extract of E. coli expressing UCP3
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... UCP3(7352)
mouse ... Ucp3(22229)
rat ... Ucp3(25708)
Categorías relacionadas
Descripción general
Anti-Uncoupling Protein 3 (UCP3) is developed in rabbit using a synthetic peptide corresponding to amino acid residues 254-267 of human UCP3, with N-terminal lysine added, conjugated to keyhole limpet hemocyanin (KLH). The uncoupling proteins (UCPs) are mitochondrial inner membrane proteins which comprises UCP1, 2, 3, 4 and 5. UCP3 displays two forms, a long mature, UCP3L and a short, UCP3S form. UCP3 is preferentially expressed in skeletal muscle and in brown adipose tissue (BAT).
UCP3 is a skeletal muscle protein that functions to uncouple mitochondrial respiration from ATP production. UCP3 regulates fatty acid and energy metabolism. This protein inhibits lipid-induced oxidative stress in the mitochondrial components of heart and skeletal muscle cells. UCP3 may also be involved in the pathogenesis of type 2 diabetes . Anti-UCP-3 antibody is specific for UCP3 in humans, rats and mice and does not react with UCP1 and UCP2. Staining of the UCP3 band by immunoblotting is specifically inhibited with the immunizing peptide.
Inmunógeno
The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids, respectively.
synthetic peptide corresponding to human UCP-3 sequence (amino acids 254-267) with N-terminal lysine added, conjugated to KLH.
Aplicación
Anti-UCP-3 antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections at 1:3,000 dilution, using protease-digested sections of human and mouse skeletal muscle). The antibody may also be used in immunoblot (approx. 34 kDa, additional weak bands may be detected in some preparations of brown adipose tissue extracts) and western blot (at 1:1,000 dilution, extract of E. coli expressing UCP3).
Anti-UCP-3 antibody produced in rabbit has been used in immunohistochemistry and western blotting.
Acciones bioquímicas o fisiológicas
The uncoupling proteins (UCPs) are considered as transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They are capable of returning protons pumped by the respiratory chain to the mitochondrial matrix. UCP3 is an active proton transporter, regulated by CoQ (ubiquinone), fatty acids or nucleotides.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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The on-off switches of the mitochondrial uncoupling proteins
Azzu V and Brand MD
Trends in Biochemical Sciences, 35(5), 298-307 (2010)
Ana Isabel Padrão et al.
Biochimica et biophysica acta, 1822(8), 1189-1197 (2012-05-01)
Hyperglycaemia-related mitochondrial impairment is suggested as a contributor to skeletal muscle dysfunction. Aiming a better understanding of the molecular mechanisms that underlie mitochondrial dysfunction in type 1 diabetic skeletal muscle, the role of the protein quality control system in mitochondria
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Koltai E, et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 303(2), R127-R134 (2012)
Nikolett Hart et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 61, 53-59 (2013-02-21)
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American journal of physiology. Regulatory, integrative and comparative physiology, 303(2), R127-R134 (2012-05-11)
A decline in mitochondrial biogenesis and mitochondrial protein quality control in skeletal muscle is a common finding in aging, but exercise training has been suggested as a possible cure. In this report, we tested the hypothesis that moderate-intensity exercise training
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