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U3383

Sigma-Aldrich

Urease Buffer Reagent

Sinónimos:

Urea colorimetric determination assay reagent

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1 VIAL
104,00 €

104,00 €

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.54

104,00 €

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descripción

When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.

Nivel de calidad

200

Formulario

powder

mol peso

545 kDa

temp. de almacenamiento

2-8°C

Descripción general

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia. [1]
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide.[2][3] It is secreted by several bacterial species, invertebrates, fungi, and plants.[3] Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections.[2] two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.[3]

Aplicación

Urease Buffer Reagent has been used:
  • to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method[4]
  • in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells[5]
  • to study the metabolic capacity of rat hepatocytes[6]

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H315 - H319 - H334

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
0000252832
SLCR3981
0000252832
SLCR3981
SLCL1109

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Gunnar Dahlén et al.
BMC oral health, 18(1), 89-89 (2018-05-20)
Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral
Kang Cheng et al.
Hepatology (Baltimore, Md.), 50(4), 1194-1203 (2009-07-29)
Tracking stem/progenitor cells through noninvasive imaging is a helpful means of assessing the targeting of transplanted cells to specific organs. We performed in vitro and in vivo studies wherein adult human hepatocytes and human fetal liver stem/progenitor cells were labeled
Yuta Enami et al.
Hepatology (Baltimore, Md.), 55(4), 1182-1192 (2011-10-14)
Organs from non-heart-beating donors are attractive for use in cell therapy. Understanding the nature of molecular perturbations following reperfusion/reoxygenation will be highly significant for non-heart-beating donor cells. We studied non-heart-beating donor rats for global gene expression with Affymetrix microarrays, hepatic
Cristian Follmer
Phytochemistry, 69(1), 18-28 (2007-08-21)
The broad distribution of ureases in leguminous seeds, as well as the accumulation pattern of the protein during seed maturation, are suggestive of an important physiological role for this enzyme. Since the isolation and characterization of jack bean urease by
Simon Svane et al.
Scientific reports, 10(1), 8503-8503 (2020-05-24)
The nickel-dependent urease enzyme is responsible for the hydrolysis of urea to ammonia and carbon dioxide. A number of bacteria produce urease (ureolytic bacteria) and are associated with various infectious diseases and ammonia emissions from agriculture. We report the first
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Preguntas

1–3 de 3 Preguntas  

  1. What's the concentration of Jack bean Urease? I can only see the concentration of buffer.

    1 respuesta

    1. As provided, this product contains 100 units per vial. The mass weight per vial is not determined.

      ¿Le ha resultado útil?

  2. How many urease units does 1 vial contain?

    1 respuesta

    1. As provided, this product contains 100 units per vial.

      ¿Le ha resultado útil?

  3. What is the volume of water used for reconstitution of 1 vial?

    1 respuesta

    1. As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.

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