Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension. Otherwise, you need to centrifuge, remove the media and then fix. Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.
TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Sinónimos:
biomass viability assay, sulforhodamine B, total biomass assay
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1 KIT
434,00 €
434,00 €
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1 KIT
434,00 €
About This Item
Código UNSPSC:
12352207
NACRES:
NA.84
434,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
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Formulario
liquid
uso
1 kit sufficient for 1,000 tests
envase
pkg of 1 kit
condiciones de almacenamiento
dry at room temperature
λmáx.
565 nm
aplicaciones
cell analysis
detection
método de detección
colorimetric
temp. de almacenamiento
room temp
Descripción general
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Aplicación
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein.[1][2] It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines.[3] Absorbance of dye is measured at a wavelength of 565nm.
Acciones bioquímicas o fisiológicas
Dye binds to cellular protein and is then solubilized in base.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
Código de clase de almacenamiento
3 - Flammable liquids
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Artículos
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
1 respuesta-
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When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
1 respuesta-
If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed. This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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