T2885

3,3′,5,5′-Tetrametilbencidina

TMB substrate, chromogenic, ≥98% (TLC), powder

• Sinónimos: BM azul, Sure Blue TMB, Sustrato TMB, TMB, Transferencia de TMB Plus
• Fórmula lineal: [-C₆H₂(CH₃)₂-4-NH₂]₂
• Número CAS: 54827-17-7
• Peso molecular: 240.34
• NACRES: NA.83
• PubChem Substance ID: 24900090
• UNSPSC Code: 12352204
• EC Number: 259-364-6
• MDL number: MFCD00007748
• Beilstein/REAXYS Number: 2808541

Propiedades

• Nombre del producto: 3,3′,5,5′-Tetrametilbencidina, ≥98% (TLC)
• Quality Segment: 200
• assay: ≥98% (TLC)
• form: powder
• mp: 168-171 °C (lit.)
• solubility: ethyl acetate: 50 mg/mL, clear to very slightly hazy
• storage temp.: 2-8°C
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Descripción

Application

La 3,3′,5,5′-tetrametilbencidina (TMB) es un sustituto no carcinógeno (prueba de Ames negativa) de la bencina, adecuada como sustrato de la peroxidasa para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color azul claro y puede leerse espectrofotométricamente a 370 o 620-650 nm. La reacción de la TMB puede detenerse con H₂SO₄ 2 M (que produce un color amarillo) y leerse a 450 nm. Reactivo sensible y específico para la detección de sangre, análisis de la hemoglobina, análisis de peroxidasa.

3,3′,5,5′-Tetramethylbenzidine has been used:

• as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2[1]
• as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
• in myeloperoxidase activity assay to quantify neutrophil

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H302,H351,H413
• pcodes: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves