SRP3083

IL-1 beta human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinónimos: Catabolin, Endogenous Pyrogen (EP),, Leukocyte Endogenous Mediator (LEM),, Lymphocyte-activating factor (LAF), Mononuclear Cell Factor (MCF)
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Análisis: ≥98% (HPLC); ≥98% (SDS-PAGE)
• formulario: lyophilized
• potencia: ≤0.001 ng/mL ED₅₀
• mol peso: 17.3 kDa
• envase: pkg of 10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• Nº de acceso UniProt: P01584
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... IL1B(3553)

Descripción

Descripción general

IL-1β (interleukin 1 β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. It is synthesized as a pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1. IL-1β belongs to the IL-1 gene family. The gene is mapped to human chromosome 2q14. Recombinant human IL-1β is a 17.3kDa protein containing 153 amino acid residues.

Aplicación

IL-1β (interleukin 1 β) human has been used to study the effect of pro- and anti-inflammatory mediators on leukotriene B4 and C4 synthesis as well as secretion in an inflamed porcine endometrium. It has been used to study the effect of cytokines on synthesis and secretion of leukotrienes from endometrial epithelial cells of pigs.

Acciones bioquímicas o fisiológicas

Both IL-1α (interleukin 1 α) and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF (fibroblast growth factor)-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. IL-1β is involved in proinflammatory and immunological responses. Some of them include enhancing IL-2 generation, and increasing circulating NO (nitric oxide) and bringing neutrophils. It also regulates gene expression of inflammatory cytokines, mesenchymal growth factors and colony stimulating factors. IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis. It brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura. It is also associated with destruction of β-cell in type 1 diabetes.

Secuencia

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Forma física

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 50mM NaCl.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable