SRP3074
IL-12p40 human
recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
origen biológico
human
recombinante
expressed in CHO cells
Análisis
≥98% (HPLC)
≥98% (SDS-PAGE)
formulario
lyophilized
mol peso
40 kDa
envase
pkg of 10 μg
técnicas
cell culture | mammalian: suitable
impurezas
<0.1 EU/μg endotoxin, tested
color
white to off-white
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... IL12B(3593)
Descripción general
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.
The interleukin-12 subunit β (IL12β) gene is localized on human chromosome 5q31–33. The encoded protein is part of IL-12, which is a heterodimer. It is mainly expressed by antigen-presenting cells (APCs).
Acciones bioquímicas o fisiológicas
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.
Interleukin-12 subunit β (IL12β) is an immunoregulatory cytokine. It is involved in pathogenic defense responses and is also suggested as an anticancer therapeutic agent. IL12B is responsible for polarizing naive CD4+ cells toward a TH1 (T helper) phenotype and inducing IFN-γ (interferon) secretion by T and natural killer (NK) cells. This protein also stimulates the differentiation of cytotoxic CD8+ cells and enhances the activity of NK cells. IL12B is linked with many autoimmune diseases, such as psoriasis, systemic lupus erythematosus, Crohn′s disease and ulcerative colitis.
Secuencia
p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Forma física
Lyophilized from 1x PBS, pH 7.2.
Reconstitución
Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Journal of Interferon & Cytokine Research, 36(1), 62-73 (2016)
Li Zhang et al.
PloS one, 10(6), e0130982-e0130982 (2015-06-24)
This study aims to determine whether the genetic polymorphisms of IL-12B gene is a susceptibility factor to Ankylosing spondylitis (AS) in mainland Han Chinese population. Eight single-nucleotide polymorphisms (SNPs) (rs10045431, rs11167764, rs3212227, rs6556412, rs6556416, rs6871626, rs6887695 and rs7709212) in the
M K Gately et al.
Journal of immunology (Baltimore, Md. : 1950), 147(3), 874-882 (1991-08-01)
IL-12 is a heterodimeric cytokine that was identified on the basis of its ability to synergize with IL-2 in the induction of cytotoxic effector cells and was originally called cytotoxic lymphocyte maturation factor (CLMF). IL-12 was also found to stimulate
M B Atkins et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 3(3), 409-417 (1997-03-01)
A Phase I dose escalation trial of i.v. administered recombinant human interleukin 12 (rhIL-12) was performed to determine its toxicity, maximum tolerated dose (MTD), pharmacokinetics, and biological and potential antineoplastic effects. Cohorts of four to six patients with advanced cancer
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