SRP0415

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Sinónimos: OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
• Número de CAS: 1644286-11-2
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Análisis: ≥80% (SDS-PAGE)
• formulario: aqueous solution
• mol peso: 59 kDa
• envase: pkg of 20 μg
• fabricante / nombre comercial: Sigma-Aldrich
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: 1.8 mg/mL
• técnicas: cell based assay: suitable
• solubilidad: water: soluble
• Nº de acceso NCBI: NM_182470
• Nº de acceso UniProt: P14618
• aplicaciones: life science and biopharma
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PKM(5315)

Descripción

Descripción general

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.

Aplicación

PKM1active human has been used in:

• cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
• oxidative stress experiments on PKM2.
• Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).

Acciones bioquímicas o fisiológicas

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H360D
• Consejos de prudencia: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable