SRP0275

PDE10A Active from rat

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinónimos: Pde10a3, Phosphodiesterase 10A, Testis-specific phosphodiesterase PDE10A2, cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: rat
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥80% (SDS-PAGE)
• Formulario: aqueous solution
• mol peso: 66 kDa
• envase: pkg of 5 μg
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_022236
• Nº de acceso UniProt: Q9Y233
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: rat ... PDE10A(63885)

Descripción

Descripción general

Rat PDE10A GenBank Accession No. NM_022236), amino acids 449-end with N-terminal GST tag, MW = 66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definición de unidad

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.