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SNC10

Sigma-Aldrich

Mission® Small RNA Purification Kit

1 sufficient for 10 preparations

Sinónimos:

microRNA Isolation Kit

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.52

uso

sufficient for 10 preparations

técnicas

DNA extraction: suitable

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Descripción general

Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Aplicación

mirPremier® microRNA Isolation Kit has been used to:
  • prepare small RNA
  • enrich small RNAfractions from the human astrocytes for small RNA sequencing and bioinformatics analysis
  • isolate small RNA from Vigna mungo tissues
  • extract miRNAs to detect the expression of miRNA-16

Características y beneficios

  • Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
  • Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Capable of extracting miRNA with high purity with no detectable large RNA.
  • Hazardous organic extractions are not involved.

Información legal

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H302 + H332,H315,H319

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
Xiaoqiong Tang et al.
Cancer management and research, 12, 2119-2125 (2020-04-14)
LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the
Ying Zhang et al.
Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
Jiayong Xie et al.
Regenerative therapy, 21, 87-95 (2022-07-06)
Diabetic nephropathy (DN) is a severe diabetic complication and podocyte damage is a hallmark of DN. The Nucleoporin 160 (NUP160) gene was demonstrated to regulate cell proliferation and apoptosis in mouse podocytes. This study explored the possible role and mechanisms
Identification of microRNA-mRNA regulatory network associated with oxidative DNA damage in human astrocytes
Nwokwu CD, et al.
ASN Neuro, 14, 17590914221101704-17590914221101704 (2022)
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