SAB5801017

Alpaca Anti-Mouse kappa HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Sinónimos: Recombinant Alpaca single variable domain secondary Nanobody
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: alpaca
• recombinante: expressed in E. coli
• conjugado: horse radish peroxidase (HRP)
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: secondary antibodies
• clon: recombinant monoclonal
• formulario: lyophilized solid
• mol peso: 14 kDa
• reactividad de especies: mouse, Alpaca
• técnicas: ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• tamaño: 0.1 mL
• color: colorless
• isotipo: VHH
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Alpaca Anti-Mouse kappa HRP single domain Antibody is designed to specifically detect mouse IgG kappa chain. It is a monovalent, single domain, recombinant antibody to mouse IgG kappa chain coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse kappa HRP single domain Antibody detects the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Especificidad

Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c

Inmunógeno

Recombinant Alpaca single variable domain secondary nano antibody

Envase

100 μg in Glass bottle

Forma física

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitución

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Almacenamiento y estabilidad

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Otras notas

No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317,H412
• Consejos de prudencia: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Aquatic Chronic 3 - Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable