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SAB5600096

Sigma-Aldrich

Anti-His-Tag Chimeric antibody, Human Monoclonal

recombinant, expressed in HEK 293 cells, clone RMH01, purified immunoglobulin

Sinónimos:

Anti-His tag antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

recombinante

expressed in HEK 293 cells

Nivel de calidad

100

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

RMH01, monoclonal
recombinant monoclonal

formulario

buffered aqueous glycerol solution

técnicas

capture ELISA: 0.1-2 μg/mL
western blot: 0.1-1 μg/mL

isotipo

IgG

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

Hexahistidine tag (His-tag) consists of four to six histidines fused genetically. It is widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC).

Especificidad

This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Inmunógeno

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins?evaluation of two anti-His-tag monoclonal antibodies
Muller KM, et al.
Analytical Biochemistry, 259(1), 54-61 (1998)
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The failures of anti-β-amyloid immunotherapies suggested that the very low fraction of injected antibodies reaching the brain parenchyma due to the filtering effect of the BBB may be a reason for the lack of therapeutic effect. However, there is no

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