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SAB4504373

Sigma-Aldrich

Anti-phospho-Cortactin (pTyr466) antibody produced in rabbit

affinity isolated antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen 61 kDa

reactividad de especies

human, rat, mouse

concentración

~1 mg/mL

técnicas

ELISA: 1:20000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

phosphorylation (pTyr466)

Información sobre el gen

mouse ... Cttn(13043)

Descripción general

Cortactin is an actin-related protein 2/3 (Arp2/3) complex–activating and filamentous (F)-actin–binding protein. This protein is coded by CTTN gene that maps to human chromosome 11q13.

Inmunógeno

The antiserum was produced against synthesized peptide derived from mouse Cortactin around the phosphorylation site of Tyr466.

Immunogen Range: 441-490

Aplicación

Anti-phospho-Cortactin (pTyr421) antibody has been used in western blotting and immunoprecipitation.

Acciones bioquímicas o fisiológicas

Cortactin controls Arp2/3-mediated actin polymerization. This protein also modulates aggressive cancer cell traits like tumor growth, cancer cell invasion and motility, and serum and anchorage-independent growth.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Anita Kumari et al.
Nature communications, 10(1), 2870-2870 (2019-06-30)
An important channel of cell-to-cell communication is direct contact. The immune synapse is a paradigmatic example of such type of interaction: it forms upon engagement of antigen receptors in lymphocytes by antigen-presenting cells and allows the local exchange of molecules
Cyclic expression of the voltage?gated potassium channel KV10. 1 promotes disassembly of the primary cilium.
Sanchez A, et al.
EMBO Reports, e201541082-e201541082 (2016)
Araceli Sánchez et al.
EMBO reports, 17(5), 708-723 (2016-04-27)
The primary cilium, critical for morphogenic and growth factor signaling, is assembled upon cell cycle exit, but the links between ciliogenesis and cell cycle progression are unclear. KV10.1 is a voltage-gated potassium channel frequently overexpressed in tumors. We have previously
Cortactin overexpression regulates actin-related protein 2/3 complex activity, motility, and invasion in carcinomas with chromosome 11q13 amplification.
Rothschild B L, et al.
Cancer Research, 66(16), 8017-8025 (2006)
Cortactin promotes exosome secretion by controlling branched actin dynamics.
Sinha S, et al.
The Journal of Cell Biology, 214(2), 197-213 (2016)

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