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SAB4301138

Sigma-Aldrich

Anti-GFP antibody produced in rabbit

affinity isolated antibody

Sinónimos:

GFP-like chromoprotein

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

27 kDa

concentración

3.2 mg/mL

técnicas

western blot: 1:1000-1:10000 (Cell Lysate)

isotipo

IgG

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

Green fluorescent protein (GFP) is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+- activated photoprotein aequorin to GFP.

Especificidad

The antibody detects transfected proteins containing GFP tag.

Inmunógeno

Full length fusion protein

Aplicación

Anti-GFP antibody produced in rabbit has been used in:
  • double immunofluorescence staining
  • immunoblot analysis.
  • immunoprecipitation.

Acciones bioquímicas o fisiológicas

Green fluorescent protein (GFP) is a reporter molecule which is used for checking gene expression and protein localization in vivo, in situ and in real time. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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David A Rhodes et al.
Frontiers in immunology, 9, 662-662 (2018-04-20)
Activation of human Vγ9/Vδ2 T cells by "phosphoantigens" (pAg), the microbial metabolite (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) and the endogenous isoprenoid intermediate isopentenyl pyrophosphate, requires expression of butyrophilin BTN3A molecules by presenting cells. However, the precise mechanism of activation of Vγ9/Vδ2 T
Green fluorescent protein as a reporter for macromolecular localization in bacterial cells.
Margolin W
Methods, 20(1), 62-72 (2000)
UGT79B31 is responsible for the final modification step of pollen-specific flavonoid biosynthesis in Petunia hybrida.
Knoch E, et al.
Planta, 247(4), 779-790 (2018)
Minna M Koskela et al.
The Plant cell, 30(8), 1695-1709 (2018-07-04)
The amount of light energy received by the photosynthetic reaction centers photosystem II (PSII) and photosystem I (PSI) is balanced through state transitions. Reversible phosphorylation of a light-harvesting antenna trimer (L-LHCII) orchestrates the association between L-LHCII and the photosystems, thus
RNA-dependent RNA polymerases of both virulent and benign rabbit caliciviruses induce striking rearrangement of Golgi membranes.
Urakova N, et al.
PLoS ONE, 12(1), e0169913-e0169913 (2017)

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