SAB4200744

Anti-BDNF antibody, Mouse Monoclonal

clone BDNF129-13, purified from hybridoma cell culture

• Sinónimos: Anti-Abrineurin, Anti-Brain-derived neurotrophic factor
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: BDNF129-13, monoclonal
• formulario: buffered aqueous solution
• mol peso: ~12 kDa
• reactividad de especies: human
• concentración: ~1.0 mg/mL
• técnicas: immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein; immunocytochemistry: suitable; immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections; immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein
• isotipo: IgG2b
• Nº de acceso UniProt: P23560
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... BDNF(627)

Descripción

Descripción general

Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS).

Inmunógeno

Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH

Aplicación

Anti-BDNF antibody, Mouse Monoclonal has been used in:

• immunoblotting
• immunohistochemistry
• immunoprecipitation
• immunocytochemistry

Acciones bioquímicas o fisiológicas

Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable