SAB4200352

Anti-DLC1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Sinónimos: Anti-ARHGAP7, Anti-Deleted in Liver Cancer 1, Anti-FLJ21120, Anti-HP, Anti-STARD12, Anti-p122-RhoGAP
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~200 kDa
• reactividad de especies: human
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentración: ~1.5 mg/mL
• técnicas: western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human DLC1.
• Nº de acceso UniProt: Q96QB1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... DLC1(10395)

Descripción

Descripción general

Deleted in liver cancer 1 (DLC1) is a candidate tumor suppressor gene, originally obtained from human hepatocellular carcinoma (HCC). It is mapped to the human chromosome location 8p21-22. The DLC1 gene is majorly expressed in normal human tissues. It contains three major functional domains, sterile α-motif (SAM), Rho guanosine triphosphatase-activating protein (RhoGAP), and steroidogenic acute regulatory-related lipid transfer (START) domains. The N-terminal region of DLC1 localizes to focal adhesions by binding to Src Homology 2 (SH2) domains on tensins.

Especificidad

Anti-DLC1(C-terminal) specifically recognizes human DLC1.

Inmunógeno

synthetic peptide corresponding to a sequence at the C-terminal of human DLC1, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Aplicación

Anti-DLC1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Acciones bioquímicas o fisiológicas

Deleted in liver cancer 1 (DLC1) interacts with tensin2, a focal adhesion protein, localized at the end of stress fibers, which plays key roles in cytoskeletal organization and cell signaling. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The focal adhesion localization of DLC1 is critical for cell motility and morphology. It is also critical for the tumor suppressor activity of DLC1 and under expressed in human hepatocellular carcinoma (HCC) and a variety of cancer types.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable