SAB4200090

Anti-Raver1 (C-terminal) antibody produced in rabbit

IgA fraction of antiserum

• Sinónimos: Anti-Ribonucleoprotein PTB-binding 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: IgA fraction of antiserum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~77 kDa
• técnicas: immunoprecipitation (IP): 5-10 μL using lysates of HEK-293Tcells overexpressing human Raver1; indirect immunofluorescence: 1:500-1:1000 using paraformaldehyde fixed HEK-293T cells overexpressing human Raver1; western blot: 1:500-1:1000 using lysates of HEK-293T cells overexpressing human Raver1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RAVER1(125950) ; mouse ... Raver1(71766) ; rat ... Raver1(298705)

Descripción

Descripción general

Raver1 is encoded by the gene mapped to human chromosome 19p13.2.

Raver1, also known as ribonucleoprotein phosphotyrosine-binding (PTB)-1, is a widely expressed multidomain protein. Raver1 is composed of three RNA recognition motifs (RRM) and both nuclear localization and nuclear export signals. It thus, shuttles between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI, a protein involved in RNA splicing of microfilament proteins.

Especificidad

Anti-Raver1 (C-terminal) recognizes human Raver1.

Aplicación

Anti-Raver1 (C-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Acciones bioquímicas o fisiológicas

Variation in the raver1 gene expression may be associated with the development of depressive disorder. In skeletal muscle, migration of of Raver1 from the nucleus to the cytoplasm facilitates differentiation of specific microfilament attachment sites.

Vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable