SAB4200087

Anti-Dicer antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Sinónimos: Anti-Dicer1, Anti-Endoribonuclease Dicer, Anti-HERNA, Anti-Helicase with RNase motif
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~218 kDa
• concentración: ~1.0 mg/mL
• técnicas: immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells; western blot: 3-6 μg/mL using HeLa cell lysates
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... DICER1(23405) ; mouse ... Dicer1(192119)

Descripción

Descripción general

Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.

Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.

Especificidad

Anti-Dicer recognizes human Dicer.

Aplicación

Anti-Dicer antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescent staining

Acciones bioquímicas o fisiológicas

Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.

Mutation in the Dicer gene causes malignancies.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable