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SAB2702136

Sigma-Aldrich

Monoclonal Anti-BRCA1 antibody produced in mouse

Sinónimos:

PNCA4, PPP1R53, PSCP, RNF53

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

6B4
monoclonal

formulario

liquid

mol peso

220 kDa

reactividad de especies

mouse, human

concentración

1.36 mg/mL

técnicas

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:500-1:3000

isotipo

IgG1

Nº de acceso UniProt

P38398

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... BRCA1(672)

Descripción general

The gene BRCA1 is mapped to human chromosome 17q21 and codes for a protein with zinc finger motif and a set of acidic residues. BRCA1 is localized to the nucleus and cytoplasm.

Inmunógeno

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Aplicación

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC, IP, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Acciones bioquímicas o fisiológicas

BRCA1-6B4 recognizes full length BRCA1, a 220 kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51. BRCA1 is predominantly expressed in proliferating and differentiating cells and contributes to cellular growth.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Phosphate-buffered saline, no preservative added.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The nuclear localization sequences of the BRCA1 protein interact with the importin-α subunit of the nuclear transport signal receptor.
Chen C F, et al.
The Journal of Biological Chemistry, 271(51), 32863-32868 (1996)
Location of BRCA1 in human breast and ovarian cancer cells.
Scully R, et al.
Science, 272(5258), 123-123 (1996)
Epidemiology of breast cancer.
Key T J, et al.
Lancet Oncology, 2(3), 133-140 (2001)
Michal Franek et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 64(11), 669-686 (2016-09-30)
DNA damage response (DDR) in ribosomal genes and mechanisms of DNA repair in embryonic stem cells (ESCs) are less explored nuclear events. DDR in ESCs should be unique due to their high proliferation rate, expression of pluripotency factors, and specific
Anuratha Sakthianandeswaren et al.
Cancer discovery, 8(8), 988-1005 (2018-06-09)
ADP-ribosylation is an important posttranslational protein modification that regulates diverse biological processes, controlled by dedicated transferases and hydrolases. Here, we show that frequent deletions (∼30%) of the MACROD2 mono-ADP-ribosylhydrolase locus in human colorectal cancer cause impaired PARP1 transferase activity in

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