S9076

Superoxide Dismutase I human

recombinant, expressed in E. coli

• UNSPSC Code: 12352204
• EC Number: 232-943-0
• NACRES: NA.54
• Número CE: 1.15.1.1 (BRENDA, IUBMB)
• Assay: ≥95% (SDS-PAGE and HPLC)
• Biological source: human
• Recombinant: expressed in E. coli

Propiedades

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE and HPLC)
• form: lyophilized powder
• mol wt: 31 kDa
• technique(s): cell culture | mammalian: suitable
• color: white
• application(s): detection
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... SOD1(6647)

Descripción

General description

Research Area: Cell Signaling
Cu/Zn superoxide dismutase (SOD1) is an intracellular antioxidant enzyme. A mature, functional human SOD1 is a relatively small (32 kDa) homodimeric metalloprotein.

Recombinant Superoxide Dismutase I is fully biologically active when compared to standard.

Application

Superoxide Dismutase I human has been used to treat THP-1 (human leukemia monocytic cell line) or human primary macrophage cells to confirm signal specificity for superoxide.

Biochem/physiol Actions

Cu/Zn superoxide dismutase (SOD1) regulates basal levels of oxidative stress arising from the production of mitochondrial and cytosolic superoxide (O2 .−). Its high cytosolic abundance makes it unique from the other two human superoxide dismutases. SOD1 was believed to be a copper (Cu) storage protein, however, the crucial role of SOD1 is to act as an intracellular antioxidant. SOD1 also initiates gene transcription following exposure to neurotoxic stimuli and modulates signal transduction pathways involving reactive oxygen species (ROS). However, it is also implicated in multiple molecular mechanisms of cytotoxicity, contributing to pathology in diseases such as heart failure, cancer, diabetes, Down′s syndrome, amyotrophic lateral sclerosis (ALS), and Parkinson′s disease.

Preparation Note

Reconstitute in H₂O to a concentration of ≥100 μg/ml. The solution can then be diluted into other aqueous buffers.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable